HiPep Columns 

C18 写真-2014-12-17-13-48-21
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Description

The founder of HiPep Laboratories has continued to research peptides and their derivatives since 1973. In the meantime, he anticipated HPLC which was introduced in bioresearch field, and invested a lot of efforts to separation, purification and analysis. We mainly used a wide variety of C18 reversed-phase silicagel columns for synthetic peptides generated by the SPPS which have 500 to 10,000 molecular-weights, also natural peptides isolated from tissue (organs). Dr. Nokihara founded HiPep Laboratories in 2002 and founded the best packing for peptide analysis, separation, analysis and preparative purification listed below.

Our targeting performances of columns:

  • High resolution, reproducibility, endurance
  • Easy migration to semi-preparative purification
  • Higher recovery in preparative purification

A column sized 20 X 150 mm can separate around 10 mg of crude peptides at one time. Purification recovery depends on crude materials and generally 30 to 50%. Purification test were done with specially synthesized peptides with varieties of packing materials with different derivatization.

Price

Product name P/N Price(without tax)
HiPep-Cadenza Caliber3mm X Length75mm HC18-375 Please feel free to ask.
HiPep-Cadenza Caliber4.6mm X Length75mm HC18-4675 Please feel free to ask.
HiPep-Cadenza Caliber2mm X Length150mm HC18-2150 Please feel free to ask.
HiPep-Cadenza Caliber3mm X Length150mm HC18-3150 Please feel free to ask.
HiPep-Cadenza Caliber4.6mm X Length150mm HC18-46150 Please feel free to ask.
HiPep-Cadenza Caliber10mm X Length250mm HC18-10250 Please feel free to ask.
HiPep-Cadenza Caliber20mm X Length150mm HC18-20150 Please feel free to ask.
HiPep-Cadenza Caliber20mm X Length250mm HC18-20250 Please feel free to ask.
HiPep-Cadenza Caliber28mm X Length250mm HC18-28250 Please feel free to ask.

All columns above have greater performances than the others in theoretical plate number, resolution, residual silanol, acid resistance and lower nonspecific adsorption. For designing, we gave priority to highly-efficient separation for peptide-related materials. All analytical columns are LCMS-capable, while we routinely use them in our own research as well as contract research.

Precautions for column handling
Please avoid excessive mechanical shock.
When you connect columns: There is a mark of FLOW on the column’s label. Please connect the column in the direction of eluent flow. When you use a wrench to set tube-in-tube, please put the wrench at the hexagonal part of its end-fitting top. Do not put the wrench at a slit on column’s pipe.
Before use insoluble material must be removed from sample solution by centrifugation in advance, and have to be filtered with membrane-filters of 0.45 μm or 0.2 μm. Floating materials or precipitates may cause pressure increase trouble by clogged columns.
Normal operation pressure: approximately 20 MPa (maximum). In consideration of solvent’s viscosity, please set flow volume not to exceed maximum pressure.
Appropriate range of column temperature is 15 – 65 ℃. Please set columns temperature, in consideration of peak shape, retention capacity and separation characteristic.
Generally silica-based columns should be used between pH 2 ? 7. Please set pH according to the purpose of your analysis. When use in alkaline-condition in the long term, column life time may shorten. Any case column life can be changed depending on the analyses conditions such as organic solvent concentration, pH adjuster composition, temperature and structure of analytes. In case of long-term storage of columns after use, please make sure to displace them in column storage solvent. Long-term storage in alkaline or acidic conditions must be avoided.
Column-wash: please remove analytes completely by higher concentration of the organic solvent. After analyses, columns have to be stored by displacing in approximately 60% aqueous acetonitrile or approximately 70% aqueous methanol solution, tightly closed and store in room temperature.

Efficient separation for structured peptides, peptides having aggregation tendency using HiPep-Intrada.
HiPep-Intrada discriminate structure of peptides and proteins. Reference: Peptide Science 2007: S.
Aimoto and S. Ono (Eds) The Japanese Peptide Society (2008) page 143-146

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Price

Product name P/N Price(without tax)
HiPep-Intrada Caliber2mm X Length150mm HiWR-2150 Please feel free to ask.
HiPep-Intrada Caliber3mm X Length150mm HiWR-3150 Please feel free to ask.
HiPep-Intrada Caliber4.6mm X Length150mm HiWR-46150 Please feel free to ask.
HiPep-Intrada Caliber10mm X Length150mm HiWR-10150 Please feel free to ask.
HiPep-Intrada Caliber10mm X Length250mm HiWR-10250 Please feel free to ask.
HiPep-Intrada Caliber20mm X Length250mm HiWR-20250 Please feel free to ask.
HiPep-Intrada Caliber28mm X Length250mm HiWR-28250 Please feel free to ask.
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