A Novel Bio-chip for Diagnostic Testing in the Next-Generation,Does not Relying on Known Biomarkers
①Rapid testing using just one drop of sample
②No labeling nor washing steps required
③Provides objective criteria for diagnoses
④Applicable, even when no established diagnostic method is available and to diseases for which no biomarkers have yet been identified
⑤Not disposable, Reusable, cost-effective
⑥No large, expensive instruments, No specialized reagents nor extensive training is required
⑦Real time on-site manner contribute to reducing regional disparities in access to healthcare
Principle of the PepTenChip®
Novel Biochip System Using the Microarray, “PepTenChip®”: A Novel Testing Method Independent from Known or Existing Substances
Conventionally disease-testing is performed to examine disease-related molecules such as (bio-markers) in biological samples, such as blood, saliva, etc. However, for many diseases, the causative substances or specific molecules that can accurately indicate the presence of disease have not yet been identified. In fact, many development for protein-chip have been withdrawn since immobilization of capturing molecules was inferior vs. solution-assays. HiPep Laboratories has successfully developed a novel biochip technology. Body fluids contain numerous compounds, although statistical data processing; multivariate analyses can allow detections without identification of disease specific compounds.
How PepTenChip® Testing Works
A large number of different fluorescent peptides (capture molecules) are immobilized on the chip to serve as sensors for analyzing biological samples (Figure 1). Peptides are small protein-like molecules, consisting of amino acids, which has structures by designing. When a drop of a biological sample is applied to the chip, various components in the sample interact with these capture molecules.
These interactions cause subtle changes in the structure, which are refracted fluorescent intensity changes. By detecting and analyzing these changes in fluorescence across the array, PepTenChip® can give characteristic molecular profiles depends on samples.
By measuring these changes in fluorescence, it is possible to determine the characteristic features of a biological sample. Because the changes in fluorescence before and after the sample is applied to the chip are extremely small, a dedicated chip and highly sensitive measurement technology are essential for accurate detection. The collective pattern of fluorescence changes obtained from a large number of peptides can be visualized as a single image, designated “Protein Fingerprint” (Figure 2). This Protein Fingerprint provides a distinctive molecular profile of the sample, enabling the characteristics of different biological conditions to be captured without relying on the detection of a specific biomarker. By statistically analyses, it becomes possible to identify differences between biological samples and distinguish between different conditions of risks of disease. To achieve the high sensitivity required for this measurement, HiPep Laboratories has also developed a novel chip substrate based on innovative substrate amorphous carbon which offers a range of advantages not found in conventional slide-glasses. The amorphous carbon has highly electric conductivity and inherently low fluorescent background, allows highly sensitive and reliable detection of subtle changes in fluorescence. The PepTenChip® microarray system provides a powerful platform for capturing and analyzing the characteristics of biological samples.
